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Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells.

机译：腺病毒感染和未感染的KB细胞中与SS-B / La核抗原相关的磷蛋白的表征。

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摘要

We have employed sera from patients with autoimmune disease to characterize the nuclear SS-B/La antigen in uninfected and adenovirus-infected KB cells. A 45,000-dalton phosphorylated polypeptide was specifically precipitated with anti-SS-B sera, and the level of phosphorylation was increased after infection. The increased phosphorylation appears to occur at the same amino acid residues phosphorylated in uninfected cells and results from increased phosphorylating activity rather than from altered enzyme specificity. A competition experiment between infected and uninfected cell extracts indicates that the antigen in the infected cell binds more strongly to SS-B/La antibodies. Fragments of adenovirus-induced virus-associated RNA as well as intact molecules complex with SS-B/La antigen and are immune precipitated with autoimmune sera.

机译：我们采用了自身免疫性疾病患者的血清来表征未感染和腺病毒感染的KB细胞中的核SS-B / La抗原。 45,000道尔顿的磷酸化多肽被抗SS-B血清特异性沉淀，感染后磷酸化水平增加。磷酸化的增加似乎发生在未感染细胞中被磷酸化的相同氨基酸残基上，这是由于磷酸化活性增加而不是酶特异性改变所致。感染和未感染细胞提取物之间的竞争实验表明，感染细胞中的抗原与SS-B / La抗体的结合更牢固。腺病毒诱导的病毒相关RNA片段以及完整的分子与SS-B / La抗原复合，并通过自身免疫血清免疫沉淀。

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作者
Pizer, L I; Deng, J S; Stenberg, R M; Tan, E M;
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年度 1983
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正文语种 en
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1. Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells. [J] . L I Pizer, J S Deng, R M Stenberg, Molecular and Cellular Biology . 1983,第7期

机译：腺病毒感染和未感染的KB细胞中与SS-B / La核抗原相关的磷蛋白的表征。
2. Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells. [J] . L I Pizer, J S Deng, R M Stenberg, Molecular and Cellular Biology . 1983,第7期

机译：腺病毒感染和未感染的KB细胞中与SS-B / La核抗原相关的磷蛋白的表征。
3. Characterization of tau antigens isolated from uninfected and simian virus 40-infected monkey cells and papovavirus-transformed cells. [J] . D T Simmons Journal of Virology . 1980,第2期

机译：从未感染和猿猴病毒40感染猴细胞和乳头病毒转化细胞中分离的Tau抗原的表征。
4. EVALUATING THE EFFECT OF FORMULATION ON THE UPTAKE OF A ZIKA SUBUNIT VACCINE CANDIDATE BY ANTIGEN-PRESENTING CELLS. [C] . Xiaoling Li, Jakub Dragun, Peter Laing, Conference on vaccine technology VI . 2018

机译：评估配方对有抗原的细胞摄取寨卡亚候选念珠菌疫苗的影响。
5. Characterization of mutant proliferating cell nuclear antigen from aphidicolin-resistant Chinese hamster ovary cells. [D] . Taylor, Kendra Natasha. 2003

机译：从抗蚜虫的中国仓鼠卵巢细胞中突变增殖细胞核抗原的表征。
6. Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells. [O] . L I Pizer, J S Deng, R M Stenberg, 1983

机译：腺病毒感染和未感染的KB细胞中与SS-B / La核抗原相关的磷蛋白的表征。
7. SS-B (La) nuclear antigen: Fast and non-degradative procedure to prepare SS-B extracts free from other nuclear antigens [O] . Bach-Elias Montse, Palau Jaume 2015

机译：SS-B（La）核抗原：制备不含其他核抗原的SS-B提取物的快速且非降解的步骤
8. In vitro Antigen-Specific Antibody Response to the Species-Specific Surface Protein Antigens of Typhus Group Rickettsiae by Human Peripheral Blood Mononuclear Cells. Generation of an Antigen-Dependent Suppressor T Cell [R] . Misiti, J., Dasch, G. A. 1985

机译：体外抗原特异性抗体对人外周血单个核细胞对斑疹伤寒组立克次体的物种特异性表面蛋白抗原的反应。产生抗原依赖性抑制T细胞

Characterization of a phosphoprotein associated with the SS-B/La nuclear antigen in adenovirus-infected and uninfected KB cells.

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